DNAvi - Video Tutorial

A demo for a clinical analysis of cell-free DNA fragmentation profiles of diagnosed vs. relapsed neuroblastoma patients based on Trinidad et al. 2023.

DNAvi - Step-by-Step tutorial

This demo part will guide you through differential fragmentation analysis for cell-free DNA in screenshots.

To start, on DNAvi's start page, click the green button to directly start an analysis.

Login
Step 1: Upload gel electrophoresis image or signal table.

On DNAvi's analysis page, the first section allows you to upload files:
Upload

Here, we will look at four samples from diagnosed vs. relapsed neuroblastoma patients in Trinidad et al. 2023. ↓ Download data

We just double-click the image to select and upload it:


DNA Table or Gel
Step 2: choose your DNA size standard

The authors used a D1000 size standard, so we will select this option in the menu.
Size Standard

Step 3: (optional) Add sample metadata

Add a row for each sample in your gel (in our case here that's four samples/gel lanes):
Add sample


We will add the information on disease and relapse status based on the original publication for our four selected samples.
add info


Also add the information on relapse status in the table (select ELBS criteria columns for this feature):
Status


and confirm the table:
Confirm

Step 4: Submit for Analysis

Great, we will select the disease status as our group information of interest for statistical comparison:
Select category


Finally, we confirm that we've read the T&C and submit. You will be asked whether to save the data on the server. This is recommended, as it will allow you to retrieve results later on in the submission dashboard.
Submit


Be patient, this may take a while. If logged in, you can also choose to save your analysis files for future use in the submissions' dashboard.
Wait

Step 5: Explore Analysis Results & Download

View your analysis results interactively in the web interface, or download a ZIP folder containing all results and plots.
Results


Done! Now you're ready to go ahead with your own data. Please don't forget to cite DNAvi and feel free to contact us if you have any feedback.

Need more help? Use our documentation.